The objective of this project is to determine the prevalence of antibiotic resistance genes in bacterial populations in impacted (close to and alongside outfall pipes and within the wastewater stream) and reference (along transect extending away from the outfalls) sediment samples around the Hollywood, FL wastewater treatment plant’s ocean outfall. Sediment and wastewater samples were analyzed for 15 different antibiotic resistant gene targets via polymerase chain reaction presence/absence assays in Southeast Florida coral reef environments.
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